METHODS OF ELICITATION OF PHENOMENON 217 



also observed an approximately 70 per cent reduction in the 

 number of leucocytes three horns after the intraperitoneal injec- 

 tion of the B. coli suspension. Similar leucopenia, ho\ve\er, may 

 be observed also in control guinea pigs injected with B. coli. 

 Postmortem examination showed severe hemcnrhagic reactions in 

 all the B. C. G. lesions. The peritoneal cavity contained a serous 

 sanguinous exudate; the adrenals appeared hyperemic and there 

 was obser\'ed stasis in the mesenteric \eins. The state of reactivity 

 of the B. C. Ct. infected sites to B. coli active principles appeared 

 approximately ten days after inoculation with B. C. G. The dura- 

 tion of reacti\ ity seemed to depend on the persistence of the 

 B. C. G. lesions. Some of the guinea pigs in which the peritoneum 

 became normal a fe^v months after the inoculation of the B. C. G. 

 did not demonstrate any state of reactivity to B. coli, ^vhilst on the 

 contrary, the state of reactivity persisted as long as several months 

 in guinea pigs in which the B. C. G. lesions failed to heal. 



Debonera, Tzortzakis, and Falchetti (1932) injected guinea 

 pigs subcutaneously ^vith 1 mgm. of virulent tubercle bacilli and 

 follo^ved this injection by an intravenous injection of 1 c.c. of 

 B. coli cultme filtrate. Four to five days after the injection of 

 the tubercle bacilli it was possible to elicit hemorrhagic necrosis 

 at the site of tuberculous foci by an intravenous injection of 1 

 c.c. of B. coli culture filtrate. The reaction showed an ischemic 

 zone surrounded by a congested ring eight hours after the intra- 

 venous injection. 



One of P. Bordet's rabbits Avhich developed a tuberctilous in- 

 fection of the eye as a result of a generalized infection, received 

 an intravenous injection of B. coli culture fdtrate. Shortly after 

 the intra\'enous injection, profuse bloody tears appeared in the 

 infected eye. 



An acti\'e preparation of B. typhosus cultme filtrate ^vas in- 

 jected by Freund (1932-33, 1934/-') intramuscularly into tubercu- 

 lous guinea pigs ^vhich pre\iously had received an intracutaneous 

 injection of bo\'ine tuberculin. Tuberculous guinea pigs ^vere in- 

 jected ^vith 1:45 to 1:500 dilution of bo\ine tuberculin follo^ved 

 one or two days later by an intravenous injection of 0.5 to 1 c.c. 

 of B. typhosus culture filtrate. At the time of the intravenous in- 

 jection, the skin at the site of tuberculin reaction was pink and 

 edematous and one hour later it became dull red. AV'ithin one 

 day it ^vas ob\'iously necrotic. The extent of the necrosis was pro- 

 portional to the severity of the tuberculin reaction (as shown in 



