METHODS OF ELICITATIOX OF PHENOMENON SIQ 



batilli in the groins. All clc\cl()pecl a severe protracted shock, 5 dying wilhin 

 two hoiiis. tncm\ minutes, and twenty hours. The guinea pigs were skin 

 lestetl the day before in iom- ditterent sites with tuberculin preparations. In 

 5 guinea pigs, 2 of Avhich survi\ed, smaller or larger hemorrhagic areas de- 

 veloped in the sites ot the skin tests. 



This author does not tliink that the hemorrhagic reaction ob- 

 served is specific either lor the tuberculous lesion or for the in- 

 flammatory reaction produced with egg white. In his oj^inion, the 

 j)ropensity to form hemorrhages in inflammatory areas of various 

 origin is probabh a symptom of the se\'ere illness of the guinea 

 pigs. It ^vill be sho\vn in Chapter ix that the interaction of non- 

 bacterial antigens ^vith homologotis antibodies is capable of pro- 

 dticing severe hemorrhagic lesions in skin sites prepared by bac- 

 terial factors endoAved ^vith high skin-preparatory potency. It may 

 be suggested, therefore, that in Dienes' experiments the skin sites 

 injected with tubercidin develop the necessary state of reactivity. 

 The interaction of the parenterally indticed egg white \vith the 

 actively acquired antibodies of guinea pigs rendered sensitive to 

 egg ^vhite, may bring about formation of reacting factcjrs capable 

 of producing reactions in the ttiberctdin prepared skin sites. 



Wadsworth and Sickles (1933) reported tiiat they were a])le to 

 elicit hemorrhagic reactions with pneiniiococt iis filtrates in areas 

 of skin infection with jjneiunococcus. 



Michelazzi (iQ^^r,^) noted that intraxenous injections of pneu- 

 mococcus cultiue filtrates elicited typical reactions in the limgs 

 of rabbits prepared by an intra\enous injection of lixe pnetmio- 

 coccus cidttnes twenty-foin- hoins pre\'iously. 



P. Bordet (ip^^Oc/) noted hemorrhagic reactions in sites of in- 

 fections xvith .Oidiimi albicans one week after the inocidation, 

 following the intraxenous injection of B. coli culture filtrates. 



Witebsky and Salm (19.^7) foimd that intraxenous injections 

 of li\e and heat-killed B. influenzae and B. Bordet-Gengou, 

 twenty-foin- hours after intradermal injection of li\e B. influenzae, 

 j)roduced lesions characteristic of the phenomenon. The state of 

 reactix'ity did not residt from infection xvith li\e B. Bordet- 

 Gengou. 



RFCAPITl'rATIOX 



The phenomenon described in this monograph ma\ be success- 

 lidl) elicited in \arious tissues and internal organs by an intra- 

 parcnc liMiial injection of the active princij^les followed b\ an 



