22() 



lOCAl. riSSUE REACTIVITY 



iiK'iUs, on the assmiiptioii llial a \iriis rcspoiisibk' Im the liimor 

 may act as a picparatoiA hutoi, the authors <^a\e a single intra- 

 venous injettioii ol the B. (oli hlliaie into saKonia-hearinp; 



d ■ 



i 



^ 



Fig. i8. toiitiol sfiies ol mice. Note t\\el\e (lav old saicoma iSo (a) : twcUe day 

 old tumor cross-sectioned (b) ; fiiiten da\ old iiimor (r) . and thiit\-fi\e day old 

 tiniior (cl) . (Shvvartzman. iggG/^.) 



guinea pigs (i.r., no intratuinoral preparatory injection ol the 

 bacterial filtrate) . Out of the 5 timior-bearing guinea pigs, 2 died 

 Avithin the following twenty-foiu' hcjtns; 2 were killed forty-eight 

 hours following the intraxenous injection and the 5th died eight 

 days after the injection. All the guinea pigs examined showed 

 extensive hemorrhage which had a tendency to extend slightly to 

 the peritoneal connective tissue. No hemorrhagic lesions were 

 observed in other organs. 



Inasmuch as the work described by Gratia and Linz (1931/) 

 was done in animals which may be considered irregularly suscep- 

 tible to the phenomenon, it still remained of considerable interest 

 to determine whether the active principles of the phenomenon 

 xvoidd be able to produce any effect upon timiors in animals 

 totally resistaiu to the phenomenon, n()tal)ly, mice, rats, etc. In 

 1932, the following experiments xvere done by me in collabora- 

 tion Avith Michailovsky. 



The timior employed was Mouse Sarcoma 180 of the Crocker 

 Institute for Cancer Research. The mortality of inocidated mice 

 xvith this tiunor xvithin the first month is approximately 45 per 

 cent. Very few mice live longer than six weeks. Sjjontaneous cines 



