228 LOCAL ILSSLE REACTIVITY 



ohsciAcd. riic tumor takes, in loo pci (ciit ol lic'tc'roj^c'iieous lines 

 ol mice. 



The bacterial liltiates emj)l()yed in the e\j)eriments al)()ut to 

 he (lesciihed weie ol lii^^li j)lienomenon-pi()(lii( ins^ potency, as 

 j)ie\i()iisly determined in rabbits, namely, "ai>ar washings" fil- 

 trates of meningococcus, 44B, (>roup 1 (1350 reacting tniits per 

 1 c.c) . The results can be summarized as follows: 



Group I. Nine mice bearing eighteen clay old tumors were each injected 

 intravenously with 0.5 c.c. of meningococcus 44B. "agar washings" filtrate 

 (No. 1700) . Tlie injection killed 2 mice Avithin twenty-four hours. At autopsy 

 there ^vas found extensive hemorrhage in the entire tumor mass and no 

 evidence of hemorrhage in any other tissue or organ. Twenty-four horns 

 after the intravenous injection, the surviving mice also showed extensive 

 hemorrhage in the tumor mass including previously healthy borders. The 

 necrotic mass hardened and separated, leaving a bed of granulation tissue. 

 However, at the borders growth reappeared. Further treatment ccjnsisted of 

 intravencjus and intraperitoneal injections of 0.5 c.c. of the same filtrate 

 ten and twelve days after the first injection, respectively. Four more mice 

 died from the injections. The surviving mice again showed hemorrhage in 

 the areas of new growth. In one mouse the tiunor regressed but began to 

 grow again. In the remaining 2 mice the necrotic mass separated, the granu- 

 lation tissue filled the bed and complete healing resvdted. The mice showed 

 no groAvth two months after timior inoculation. Their general condition Avas 

 excellent. One mouse was killed at this time. No growth was discovered in 

 any organ. 



Group Ila. (Four mice.) Mice I and IV received each intravenous injec- 

 tions of 0.25 c.c. of filtrate No. 1700 on the eighteenth, twentieth, and 

 t\\cnty-third days of timior growth. Mice II and III received in addition in- 

 jections on the thirtieth and thirty-foiuth days. Extensive hemorrhagic necro- 

 sis was evident in the tiunor masses of all the treated mice within twenty- 

 four hours after the first injection. The tumor of Mouse I was gradually 

 reduced in size until on the thirty-fifth day after tumor inoculation there 

 was no timior left. The healing proceeded uneventfully. In Mouse II the 

 tiunor receded but the animal died on the thirty-fifth day after tumor inocu- 

 lation. Autopsy showed grossly no tumor growth and no metastasis. The 

 tumor of Mouse III regressed after the first three injections but later in- 

 creased in size. The mouse died on the thirty-first clay of tumor gro^vth. 

 The tumor of Mouse IV completely disappeared. The mouse appeared com- 

 pletely free of growth on the thirty-fifth day after tumor inoculation. 



Groiip lib. (Four mice.) Mice V, VIII received each intraperitoneal in- 

 jections of 0.25 c.c. of filtrate No. 1700 on the eighteenth day of tumor 

 growth. Mice VII and VIII died 14 and 48 hours after the injection, respec- 

 tively. The autopsy showed extensive hemorrhagic necrosis of the tumors, and 

 no lesions in other organs. Mice V and VI. which showed a similar picture 

 tAventy-four hours after the injection, developed large tumors later. 



Group lie. (Four mice.) Mice IX-XII received each intravenous injections 

 of 0.5 c.c. of filtrate No. 1700 on the eighteenth, twentieth, and twenty-third 



