240 LOCAL ILSSUE REACTIVITY 



piiiuiplc's prodiKcd ])i oiiouiufd licnion liases in llu' tiiiiiois. 

 Many of the mice died but in the suivixors some tumors recefled 

 and grew ai>ain, while others receded (omplelely. 



Sliear cone hides ihal the j)ositi\'e resuhs obtained with \arious 

 non-bacterial substances are inbecjuent and cannot be rcjjroduced 

 with regularity. The effect of the aetive principles oi the phenom- 

 enon is more consistent and may l)c obtained with a higher degree 

 of regularity. It may be jM)inted out here, however, tliat inasmuch 

 as tiie actixe jjrinciples of the jshenomenon are siiliject to fluctua- 

 tions in potency on storage, it is necessary to ascertain the titer 

 of the preparation in ral)bits beh)re using it in the xvork on 

 tumors. 



Since it is cHfRcult to of)serve xvith certainty xvhether mild 

 hemorrhage fiad been produced in subcutaneous ttniiors without 

 ]<.illing" the animals and excising tlie tumor, Andervont (1936) 

 implanted tumors within the skin ovei the abdomen of mice. The 

 implantatic:)!! of tumc:»rs into the skin xvas accompanied by him 

 by using the following technique: 



The hair over the abdomen xvas shaved twenty-four horns pre- 

 vious to the inoculation, tumor tissue free of necrotic material 

 was passed through a fine mincing machine and inoculated by 

 using a 1 c.c. tidoerctdin syringe and an 18 or 20 gauge needle. 

 The needle xvas inserted just beneath the skin so that its bevel 

 was plainly visible and a small amoiuit of the minced tumor xvas 

 expelled fiom the syringe. All tumc:»rs implanted in this manner 

 grew in the cutaneous site. The use of skin tumors as test objects 

 proxed to be of value in microscopic studies of the reaction tak- 

 ing place within the tmnor. In Andervont's studies tiuiiors xvere 

 remcjved at half hour intervals after injection of the filtrates. The 

 earliest reaction began about two hours alter injection of the fil- 

 trate and cc^nsisted of an accumidation c^f xvhite blood cells xvithin 

 the capillaries of the tmnor. This xvas followed by a pronounced 

 and rapid increase in the nimiber of red blood cells imtil the 

 vessels became dilated and rupttned, causing a diffuse hemor- 

 rhage throughout the tumor. There appeared sxvelling of some 

 tumor cells prior to the hemorrhage indicating that the bacterial 

 jjroducts may have some direct effect upon the tmnor cells. This 

 is consistent xvith the previously mentioned observations of Apitz. 



In the studies of Andervont sarcoma 37 and sarcoma 180 showed 

 pronoimced variations in their susceptiloility to B. coll culture fil- 

 trates Avhen groxvn cutanec^usly in different lines of pine strain 



