2|() LOCAL rissuF. RKAcrivriY 



B. cnic) ihdi.s was isolated Iroin llic heart blood ol a luinoi- 

 bearing mouse ol ilie passage made on fan. 25, i9^^5 (Crocker 

 generation -iVylV) . Every second and later e\ery third day the 

 strain Avas j)assed through ])lain broth containing B. ciilci ilidi.s 

 rabbit set um in 1:10 dilution. Alter nineteen passages a rough 

 colony ^vhi{ h sell-agglutinated in plain broth and in o.cSr, per 

 cent solution of sodiiun chloride was tested tor xirulence in 

 normal mice (strain rough 3H) . Three stock mice were inocu- 

 lated Avith tmdiluted twenty-tour hoiu" old broth cidture and 1:10, 

 1:50, and 1:100 dilutions thereof. The observations were made 

 for a period of five days. While most of the mice inoculated with 

 undiluted broth culture and broth (iilture diluted 1:10 died dur- 

 ing the first three days, dilutions 1:50 and 1:100, ^vitli two ex- 

 ceptions, failed to kill the mice tested. Slajjhylococciis (iiirciis 

 (6H) ^vas isolated from the heart's blood of a tumor-bearing 

 mouse. 



The B. cntcritidis liltrate (T.i()9o) \vas an "agar washings" 

 filtrate. The Staphylococcus ainciis filtrate was a filtrate of a three 

 day old ])lain broth culture. Both filtrates ^vere tested for their 

 ability to elicit the phenc:)menon of local skin reactivity to bac- 

 terial filtrates in the rabbit. The B. enteritidis filtrate contained 

 500 reacting units. Slaj/l/ylococcu.s ((urcu.s j^roduced no effect even 

 in a dose of 3 c.c. per kilogram of body weight. 



Broth cultures oi Stal^hyJococcus aureus aud culture filtrates of Sfaj)liyIo- 

 coccus aureus exerted no inllueuce on the development ol sarcoma 180. 



An eighteen hoin- culture ol the rough strain of B. eiUerilidis diliUed 

 1:100 was injected intravenously into 20 mice liearing t\vel\e day old 

 tumors. Severe hemorrhagic lesions were observed in 1 1 mice, and mild 

 hemorrhagic lesions, in 4 mice, twenty-four hours after the intravenous in- 

 jection. Seven of these tumors completely regressed within ten clays after the 

 injecticju. Of the ^^ mice that ^vere alive twenty days after the injection one 

 had no tumor, one showed a tiny necrotic tumor and one had an actively 

 growing tumor. 



Ten days after inocidation with the rough strain of B. enleriiuUs 2 mice 

 were killed for bacteriologic examination. /}. euteriluUs was isolated from 

 the liinior tissue, heart blood, peritoneal and j^leuial lluids, and liver of 

 both mice. The strain was ajjparentlv the same as the one injected, since 

 it formed distinctly rough colfjnies on plain agar and gave spontaneous ag- 

 glutination in plain Ijroth and in 0.85 per cent solution of sodium chloride. 



Histologically, the hemorrhagic tumor sectioned twenty-four hours after the 

 injection of B. enteritidis (Fig. 25) , showed a zone of necrosis and hemor- 

 rhage sharply demarcating the \ iable tumor tissue. The "agar washings" 



