268 LOCAL TLSSUE REACTIVLIY 



loi li\c weeks and obtained twenty-four hoins alter the last in- 

 jection: (2S) ( liilied delibrinated ral)l)it sera, 2 e.c.; (29) ox sera, 

 1 C.C.; (30) d()(> sera, 1 e.c. 



P. Bordet (i()'^r)/>) failed to elicit reactions in j)rcj)ared skin 

 sites by intravenous injections ot "electrarool," tri-(al(iinn phos- 

 ])liate and neosahaisan. According to Cope and Howell (19^51), 

 egg white, diluted bile, ascitic fluid, brotii and spinal fluid, are 

 de\oid of reacting potency. Sickles (1931, 1934) failed to elicit any 

 reactions in skin sites prepared by bacterial filtrates by means of 

 intra\enous injections of Hitchens' mediinii (h)!^), galactose 

 (1 pcv cent in sugar free broth) , 10 per cent gelatine, horse seriuii, 

 India ink \vashed and resuspended in salt solution and pectin. 



Plant (1934) failed to oljtain reactions ^vith horse, sheep, ox, 

 swine, and guinea pig sera. Hmnan serum not older than two days, 

 however, in doses of 3 c.c. per kilo of body Aveight, gave positive 

 results. Tiic actixity ajjparently disappeared after a few days of 

 storage and coidd be partially removed by heating" at 56° for I/2 

 hoiu". 



Irregular reactions (in approximately one-third of rabbits 

 tested) xvere obtained by me when guinea pig or hmnan deli- 

 brinated blood was injected in doses of 3 c.c. or more per kilo of 

 body weight. A higher percentage of positive results was ob- 

 tained by Rid)in xvitii doses as great as 10 to 12 c.c. per rabbit. 



On a few occasions as little as 2 c.c. of lunnan seriun xvas able 

 to elicit the phenomenon. The activity of the serum either disap- 

 peared shortly after storage or the new animals tested with it were 

 totally resistant to this principle. Additional experiments ^vere 

 not done to determine this point. A nimiber of other normal sera 

 tested in amotuits of 1 to 2 c.c. per kilo of body ^veight, gave con- 

 sistently negative results. 



The reasons for the toxicity of some of the animal sera xvill be 

 discussed in Chapter x. It is obvious, however, that these results 

 cannot invalidate to any extent the observations on the activity of 

 the serimi+antiserum mixtures inasmuch as in all the experiments 

 described tlie inability of the ingredients by themselves to elicit 

 any reactions in the doses employed was always ascertained. 



In the coinse of experiments xvith a niunber of sidjstances 

 Sickles (1931, 1934) noted that the local reaction followed the in- 

 travenous injection of agar twenty-foiu' horns after intracutaneous 

 injection of toxic filtrate of meningococcus. 



When agar in the form of Hitchens' medium (1916), granu- 



