342 LOCAL TLSSUE REACTIVITY 



not be (oiiclated with any j)lia,se oi the jihenomeiioii and oc- 

 curred in all groups of rabbits. 



//. Coagulation Time. (Blood obtained by means ot cardiac 

 bleeding was put immediately into two test tubes, i c.c. each. 

 The test tubes were shaken every one-half minute until the blood 

 coagulated.) The differences in coagulation time were negligible 

 in all groups of rabbits except those receiving the antigen-[-anti- 

 body combinations as reacting factors, where increases occurred 

 more markedly in rabbits showing reactions. The initial coagu- 

 lation time determined before any injections in these rabbits 

 was on the whole greater (by about ten minutes) than in other 

 rabbits. Since all of the antigen-f-antibody raljbits were done in 

 the summer time, this was thought to be due to a seasonal 

 variation. 



///. Clot Retraction Ti?ne. The clot retraction time was from 

 one to two hours in all groups of rabbits. No significant differ- 

 ences were observed. 



IV. Fragility Tests. Hemolysis took place with 0.56 to 0.4 per 

 cent of NaCl. No significant differences were observed. 



V. Sedimentation Time. There was generally observed-a slight 

 decrease in sedimentation time iir successive determinations on 

 the same rabbits, in all groups except in the antigen-|-antibody 

 group (done in the summer time) where the sedimentation rate 

 remained about the same. Decrease in the sedimentation rate of 

 other groups could not be correlated ^vith any phase of the phe- 

 nomenon but with other successive bleedings and hence, possibly 

 with some anemia. 



[7. Lactic Acid Serum Coagulation Time. (Addition of 0.2 c.c. 

 of lactic acid to 1 c.c. of serum.) Method used by P. Bordet. Tlie 

 coagulation time ranged from seven to ten days. There "v\'as gen- 

 erally a decrease of the coagulation time follo^ving the phenom- 

 enon, most marked in serimi taken t^venty-four hours after the 

 intravenous injection (two to four days less than normally) . P. 

 Bordet obtained similar results after the intravenous injection of 

 active filtrate alone. 



VH. Hemoglobin Deterntiiiations (Sahli) . There "was found 

 a General decrease in successive determinations not associated ^vith 

 any phase of the phenomenon in all rabbits. 



VIIL Count of Red Blood Cells. The changes generally fol- 

 lowed changes in hemoglobin. 



IX. Blood Platelet Counts. (Method: 3.8 per cent citrate mixed 



