IMMUNOLOGICAL RELATIONSHIPS 343 



with blood in red blood cell pipette.) There occurred a slight 

 decrease in successive cotuits in all groups of rabbits. There was 

 a suggestive association of the phenomenon ^vith a considerable 

 drop in the niunber of blood platelets in only tAvo rabbits in 

 Avhich positi\e reactions followed the injection of meningococcus 

 "agar ^vashings" filtrate. Positive reacting animals injected with 

 other bacterial filtrates did not sho^v any significant decrease in 

 blood platelets. 



X. White Blood Cells Counts. In confirmation of P. Bordet's 

 observations there ^vas observed a leucopenia following intra- 

 venous injections of bacterial filtrates in positive and negative 

 animals and Avhere the bacterial filtrate only ^vas injected intra- 

 venously. In a few instances of the antigen-j-antibody rabbits 

 there ^vas a decrease in the ntmiber of ^vhite blood cells follo"\ving 

 the second injection of horse serum. 



XI. Differeittidl Count of White Blood Cells. The total number 

 of neutrophiles decreased in rabbits showing the phenomenon to 

 B. typhosus filtrate; in rabbits receiving the intravenous injection 

 of filtrates alone; and in a few of the antigen-)-antibody rabbits, 

 following the second injection of horse serum Avhere no intra- 

 dermal injection of bacterial filtrate was made. The ninnber of 

 neutrophiles increased slightly or remained about the same in all 

 other groups of rabbits except ^vhere the antigen-)-antibody com- 

 bination Avas used for the elicitation of the phenomenon. In these 

 rabbits the increase observed fotu" hoins after the pro\ocative 

 intravenous injection Avas the greatest. The total number of 

 lymphocytes generally decreased but there was no association 

 Avith any particular phase of the phenomenon except that it did 

 not seem to occur following the intradermal injection alone. No 

 significant changes were observed in the cotnits of other Avhite 

 cells. 



It can be concluded from the observations of Mintz that no 

 blood changes may be correlated with the phenomenon itself Avith 

 the possible exception of a decrease in blood platelets after the in- 

 jection of meningococcus "agar washings" filtrates. 



2. Accordins^ to Gratia and Linz, hemorrhaoic reaction of the 

 phenomenon protects guinea pigs and rabbits against anaphy- 

 lactic shock and anaphylactoid reactions produced ^vith serimi 

 treated wdth agar of P. Bordet. The extreme irregularity and diffi- 

 culty of production of anaphylactic shock in rabbits, already 

 mentioned before, make it impossible to draw any conclusions 



