370 LOCAL TLSSUE REACTIVITY 



7. P;iliciii J. R. Dining llic (illli day ol the disease ol a moderately severe 

 inledion. 



Heated and unheated specimens of urine were used im tlie 

 experiments. The samj)les ^vere injected into 2 to ■■>, skin sites ol 

 rabbits in a dose ol 0.2 c.c. Each specimen ol mine was tested 

 in 1 to 2 ral)bits. Some rabbits were also injected at other sites 

 with acti\e B. typhosus culture filtrate. Twenty-four hours later 

 all the rabbits received intravenously a single injection of B. 

 typJiosus cultme filtrate in a dose of 3 to 5 c.c. The phenomenon- 

 prodticing potency of this filtrate w^as ascertained prior to the 

 experiments. T^vo rabbits died twelve to twenty-four hours after 

 the intravenous injection of the toxic filtrate or at the time ^vhen 

 they showed severe hemorrhagic and necrotic reactions. For this 

 reason the results of experiments ^vith all the rabbits could be 

 taken into consideration. The readings ^vere as follows: 



Skin sites prepared with the boiled and non-boiled mine of 

 patients W. J.. T. T., and D. W., and also those prepared with 

 B. typhosus culture filtrate, showed severe hemorrhagic necrosis. 

 Skin sites prepared ^vith the urine of patients G. J., Z. E., R. A., 

 and J. R. ^vere entirely negative. 



In later experiments the skin of rabbits prepared ^vith speci- 

 mens of urine derived from cases of normal people and those 

 suffering" from grippe, bronchopneimionia, certain bacteremias 

 and Werlhof's disease, ahvays gave negative results. 



This aiuhor also attempted to determine ^vhether the active 

 principles of the phenomenon cotdd be demonstrated in the 

 sweat of typhoid fever patients. Patients were given a preparation 

 known as Causyth (phenyl-pyridin-sulfonic-pyrazolon) in a dose 

 sufficient to reduce temperature for a short period of time from 

 40-41° c. to normal. The fall in temperature was accompanied 

 by profuse perspiration. The s^veat collected in test tubes ^vas 

 injected into the skin of rabbits. Twenty-four hotns later the rab- 

 bits received B. typJwsus ciUture filtrates intravenously. The re- 

 sidts ^vith the specimens of () patients ^vere negatixe. In addition, 

 however, the sweat of 2 severe cases of typhoid fever collected in 

 the above manner was concentrated by evaporation. The concen- 

 trated sweat of one patient gave strong reactions whilst only 

 weakly positive reactions were obtained with the second concen- 

 trated specimen. 



Stolyhwo concluded that the active principles of the phenom- 



