VIII 



Physiology 



Nutritional requirements of Protozoa 

 Pine cultures as material for research 

 General types of nutrition 

 The determination of food requirements 

 Autotrophic nutrition 

 Mineral requirements 

 \'itamin requirements 



Thiamine 



Riboflavin 



The pyridoxine complex 



Pantothenic acid 



Nicotinic acid 



Biotin 



Pteroylglutamic acid and /)-aminohen- 

 zoic acid 



Nucleic acid derivatives 



Ascorbic acid 



Sterols 



Hematin 



Vitamin Bij (cyano-cobalamin) 



Protogen 



Biosynthesis of vitamins 

 The requnements of various groups 



Cryptomonadida 



Phytomonadida 



Euglenida 



Protomastigida 



Trichomonadida 



Sarcodina 



Ciliates 



Oxygen relationships and oxidations 

 Ecological distribution 

 Oxidation-reduction potentials 

 Oxygen consinnption 

 Respiratory quotients 

 Oxidations 



The cytochrome system 



Pyridine nucleotide enzymes 



Diphosphothiamine enzymes 



Flavoprotein enzymes 



Pyridoxine enzymes 



Peroxidase and catalase 



Glutathione 



Pantothenic acid enzymes 

 Adenosine phosphate system 

 Tricarboxylic acid cycle 



Digestion 



Food vacuoles 

 Digestion of proteins 

 Digestion of carbohydrates 

 Digestion of lipids 



Nitrogen metabolism 



Carbohydrate metabolism 



Synthesis of carbohydrates and lipids 



Contractile vacuoles in hydrostatic regula- 

 tion 

 The vacuolar cycle 



Growth of Piotozoa 



Individuals and populations 



Initial stationary phase 



1 he lag phase 



Phase of logarithmic growth 



Phase of negative growth acceleration 



Phase of maximal density 



Phases of death 



Size of the inoculum in relation to 

 growth 

 Initial pH of the culture medium 

 Temperaluie 

 Light and darkness 

 Effects of certain toxins and venoms 

 Effects of certain therapeutic drugs 

 Effects of carcinogenic hydrocarbons 



Effects of irradiation 



Locomotion 



Amoeboid movement 

 Flagellar locomotion 

 Swimming in ciliates 



Responses to stimuli 

 Responses to light 

 Responses to electric current 

 Responses to temperature 



Literature cited 



428 



