COLLECTION, CULTIVATION, OBSERVATION 713 



Peranema, Chilomonas, Astacia and other colorless flagellates. — A 

 number of culture fluids have been advocated. A simple yet satis- 

 factory one is as follows: Fill a finger bowl with about 150 cc. of glass 

 distilled water and place 4 rice grains on the bottom. Let the dish 

 stand for a few days, and then introduce with a pipette a number of 

 desired flagellates from a mass culture into it. Cover the bowl and 

 keep it at about 20° C. 



Mast (1939) used the following media for Chilomonas Paramecium. 

 (a) Glucose-peptone solution: 



Peptone 8 gm. 



Glucose 2 gm. 



Water 1000 cc. 



Amoeba proteus and other freshwater amoebae. — Fill a finger bowl 

 with 200 cc. of glass distilled water, and place 4 rice grains. After a 

 few days seed with amoebae (p. 710), add about 5 cc. of Chilomonas 

 culture, and cover the bowl with a glass cover. In about two weeks 

 a ring of amoebae will be found around each rice grain, and if Chilo- 

 monas do not overmultiply, the amoebae will be found abundantly in 

 another two weeks. If properly maintained, subcultures may be 

 made every 4-6 weeks. Chalkley (1930) advocates substitution of 

 the plain water with a salt solution which is composed of 



NaCl 0.1 gm. 



KCl 0.004 gm. 



CaCla 0.006 gm. 



Glass distilled water 1000 cc. 



