REPRODUCTION 



133 



desmose. From the posterior end of each centriole; astral rays extend 

 out and the spHt chromosomes form loops, pass through "tangled 

 skein" stage, and emerge as 26 chromosomes. In the metaphase, the 

 equatorial plate is made up of V-shaped chromosomes as each of the 



Fig. 60. Development of spindle and astral rays during the mitosis in 

 Barbulanympha, X930 (Cleveland), a, interphase centrioles and centro- 

 somes; b, prophase centrioles with astral rays developing from their distal 

 ends through the centrosomes; c, meeting of astral rays from two cen- 

 trioles; d, astral rays developing into the early central spindle; e, a later 

 stage showing the entire mitotic figure. 



split chromosomes are still connected at one end, which finally be- 

 comes separate in anaphase, followed by reformation of two daugh- 

 ter nuclei. 



