GENERAL SYSTEMATIC BACTERIOLOGY 309 



The cell metabolism is not primarily bound up with hydrogen sulphide or other 

 sulfur compounds, the cells in consequence containing neither sulfur granules nor 

 baeterio-purpurin. The cells apparently do not possess a well-organized or well- 

 differentiated nucleus. The cells are usually minute and spherical, rod-shaped 

 or spiral in shape, in most genera not producing true filaments; the filaments 

 when formed not sheathed, and frequently branching, thus being differentiated 

 from the iron bacteria. The cells may occur singly, in chains or other groupings. 

 The cells may be motile by means of flagella, or non-motile ; they are never notably 

 flexuous. Cell multiplication occurs always by transverse, never by longitudinal 

 fission. Some genera produce endospores, particularly the rod-shaped types. 

 . . . . Chlorophyll is absent, though the cells may be pigmented. The cells 

 may be united into gelatinous masses, but never form motile pseudo-plasmodia nor 

 develop a highly specialized cj'st-producing fruiting stage, such as is characteris- 

 tic of the Myxobacteriales. 



Castellani and Chalmers (1919, p. 924) use the simple definition, 

 " Schizomijcetes which contain neither sulphur nor baeterio-purpurin." 

 They include five families, Coccaceae, Bacillaceae, Spirillaceae, Chlamy- 

 dobacteriaceae and Mijcobacteriaceae. 



In the final revision Winslow et al. (1920, p. 200) emended the de- 

 scription as follows: 



The order Eubacteriales includes the forms usually termed the true bacteria, 

 that is, those forms which are considered least differentiated and least specialized. 

 The cell metabolism is not primarily bound up with hydrogen sulphide or other 

 sulphur compounds, the cells in consequence containing neither sulphur granules 

 nor baeterio-purpurin. The cells apparently do not possess a well-organized or 

 well-differentiated nucleus. These organisms are usually minute and spherical, 

 rod-shaped or spiral, in most genera not producing true filaments; and rarely 

 branching. The cells may occur singly, in chains or other groupings. They may 

 be motile by means of flagella, or non-motile ; but are never notably flexuous. Cell 

 multiplication occurs always by transverse, never by longitudinal fission. Some 

 genera produce endospores, particularly the rod-shaped types. Conidia not 

 observed. Chlorophjdl is absent, though the cells may be pigmented. The 

 cells may be united into gelatinous masses, but never form motile pseudoplas- 

 modia nor develop a highly specialized cyst-producing fruiting stage, such as is 

 characteristic of the Myxobacteriales. 



Heller (1921, p. 449) makes this the first order of the class "Euhao 

 terieae," with the definition: " Euhacterieae whose cells are never 

 in sheathed filaments. Conidia not observed. Free iron, sulphur, 

 or bacteriopurpurin never present. MultipKcation alwaj^s occurs 

 by transverse fission. (Committee.)" 



Eubacterieae. A subtribe of bacteria proposed by Trevisan (1879, 

 p. 136) with the following diagnosis: "Cellulae liberae vel in statu 

 quiescente in colonias mucosas indeterminatas consociatae." It is 



