GENERAL SYSTEMATIC BACTERIOLOGY 385 



plants. Complex proteins usually required. As a rule strongly aerobic (except 

 for some species of Actinomyces and the genera Fusiformis and Leptotrichia) , and 

 oxidative. Growth on culture media often slow; some genera show mold-like 

 colonies. 



In 1920 the Committee included this family in the order Actinomy- 

 cetales with the description: 



Parasitic forms. Rod shaped, frequently irregular in form but rarely fila- 

 mentous and with only slight and occasional branching. Often stain unevenly 

 (showing variations in staining reaction within the cell) . No conidia. 



Castellani and Chalmers (1919, p. 1040) note that this family (cred- 

 ited to Miehe 1909) may be placed as the second family of Micro- 

 siphonales Vuillemin with the definition, "Microsiphonales without a 

 mycelium," and including the two genera Mycobacterium and Coryn- 

 ebacterium. 



These authors, however, themselves place the family as the fifth 

 in the order Euhacteriales, and credit the name to Chester 1901. They 

 follow Chester also in reducing Corynehacterium to synonymy with 

 Mycobacterium. 



Bergey et al. (1923, p. 372) follow the Committee, including the 

 family as the second in the order Actinotnycetales. 



Mycobacterieae. A tribe named by Buchanan (1918, p. 55) with 

 the following description. 



Rod-shaped organisms occasionally showing a tendency to branching, acid- 

 fast, Gram-positive, non-motile, without spores. 



A single genus is included, Mycobacterium Lehmann and Neumann. 



Mycobacterium. A generic name proposed by Lehmann and Neu- 

 mann (1896, p. 363) with the organism of tuberculosis as a type, Myco- 

 bacterium tuberculosis. It is included in their family of the Hyphomy- 

 cetes. In the translation of the second edition (1901, p. 128) the de- 

 scription reads: 



Rods stain with usual staining solutions with difficulty or generally not at all. 

 Stain by the tubercle bacillus method, i.e., it is acid resisting. Clubbed swelling 

 of the ends in cultures rare, in tissues somewhat more often. 



In this edition is found the following statement regarding the priority 

 of Sclerothrix: 



Since we proposed this name in the first edition, we have seen that Metschni- 

 koff (Virchow's Archiv, 113, p. 70. 1888), who first recognized the peculiar position 



