GENERAL SYSTEMATIC BACTERIOLOGY 439 



which act largely on proteins and bring about putrefactive changes. 

 The family is included in the order Peritrichinae, It contains the 

 following genera: Putrihacillus and Botulobacillus. 

 Putriclostridium. Orla-Jensen (1921, p. 273) states: 



The use of the term Clostridium as a generic name presents the inconvenience 

 that under the same we must group together not only the Clostridia but also the 

 plectridia. The division into true butyric acid bacteria {Butyriclostridium), 

 the requirements of which in regard to nitrogenous nutriment are very moderate 

 (they are able to assimilate even the nitrogen of the air) and anaerobic, putrefy- 

 ing bacteria (Putriclostridium) seems natural to me. 



Putrificoideae. A name given by Heller (1921, p. 550) to the second 

 subfamily of the Clostridiaceae with the following description: 



Clostridiaceae which on meat medium produce after twenty days' incubation 

 at 37° under vaseline a reaction of pH 7.1 or a more alkaline reaction, the reaction 

 being read after the culture had been boiled. 



Eleven genera are included: Seguinillus, Regillus, Robertsonillus, 

 Nicollaierillus, Martelillus, Recordillus, Tissierillus, Putrificus, Ermen- 

 genillus, Metchnikovillus and Weinbergillus. 



Putrificus. A generic name proposed by Heller (1922, p. 27) with 

 the following description. 



Putrificoideae that do not attack sugars. Slender Gram-negative or Gram- 

 positive rods with oval terminal spores. Colonies in deep agar have radiate 

 periphery and opaque center. Putrefactive organisms found in soil and wounds . 



Type species P. Bienstocki (Bacillus putrificus Bienstock) as defined by Bien- 

 stocic (1906). References; Bienstock (1884, 1901, 1906), Klein, Rodella (1905). 



Pyobacillus. A name used by Koppanyi (1907, p. 429) to designate 

 his Pyobacillus capsulatiis cuniculi or Bacillus pyemiae cuniculi. The 

 organism was isolated from rabbits. It is a non-motile capsulated 

 rod, grows on weakly alkaline nutrient media only if at same time 

 exudate is carried over on to the nutrient medium, or serum, or serum 

 agar. Protein-rich substances necessary for growth. No growth on 

 gelatin, room temperature not suitable. It probably is to be regarded 

 as non-valid, as binomial nomenclature is not used. 



Pyobacterium. A generic name used by Kiittner (1895, p. 760), 

 for the species Pyobacterium Fischeri. The organism was found in an 

 epigastric abscess. It is closely related to Bacterium coli commune, 

 but is more slender, forms colonies pure white in color and is actively 

 motile. Milk is slowly coagulated with flocculent curd. It ferments 



