524 GENERAL SYSTEMATIC BACTERIOLOGY 



According to Trevisan (;1885, p. 83), Pacini (1854, p. 40) used the 

 designation Vibrio cholerae for the organism causing Asiatic cholera, 

 but a search of the original paper fails to show it. The nearest ap- 

 proach is the follov,?ing statement: 



Finalmente notero che nel fluido intestinale trovai ancora una grandessima 

 quantita di vihrioni; i quali, attesa la loro estrema tenuita, possono facilmente 

 passare inosservati, quando siano dispersi in una certa quantita di fluido. Questi 

 vibrioni avevano una lunghezza di 0.0020 a 40™"', ed un diametro di 0.0005 a 

 ymm-- mentre avevano qualche simiglianza al Bacterium termo di Dujardin. 



Cohn (1854) concluded the Bacterium termo of Dujardin w^ the 

 Vibrio lineola of Ehrenberg, and a result of his study of the organism, 

 placed it in the plant kingdom. 



Rabenhorst (1865, p. 7J.) gives the following generic diagnosis: 

 ^'Trichomata abbreviata filiformia, plus minus distincte articulata, 

 motu proprio, plerumque flexuoso praedita; cytioplasma pallidum, 

 interdum dilute seruginosum, coeruleum vel caesium." He lists seven 

 species, three described by Ehrenberg and four by Mueller. 



Cohn (1872, p. 178) emended the definition of the genus. The or- 

 ganisms are characterized by the wavy motion of the filaments, which 

 by their rotation give the appearance of sinuous motion, and consti- 

 tute a transition to the spiral bacteria. The two species named are 

 Vibrio rugula and V. serpens. 



Cohn (1875) included the genus in his tribe Nematogenes. Luerssen 

 (1879, p. 25) used the same definition and species. Trevisan (1879, 

 p. 148) gave the following definition "Somatia cylindrica, articulata, 

 valida, abbreviata, undulato-flexuosa." The two species listed are 

 V. serpens and V. rugula. This genus is included in the subtribe 

 Euvibrionieae. Magnin (1880, p. 92) lists the same species. Lehmann 

 ;and Neumann (1901) credit Vibrio cholerae to Buchner (1884). Van 

 Tieghem (1884) defined the genus as made up of spiral cells, promptly 

 dissociating. Zopf (1885, p. 61) states "Faden schraubig, in den 

 langeren oder kurzeren Gliedern Sporenbildung." Trevisan (1885, 

 p. 83) replaced this with Pacinia with Koch's comma bacillus as the 

 type. Hueppe (1886) included forms with spiral cells, which produce 

 endospores and change the cell form when spores are produced. Flugge 

 (1886) states that this genus differs from Spirillum in that the spirals 

 are not as pronounced. Maggi (1886, p. 176) included Vibrio as 

 the first genus of his order Ophidobacteries. Schroeter (1886, p. 166) 

 introduced Microspira with Koch's comma bacillus as the type. Hans- 

 girg (1888, p. 264) makes this the first subgenus of the genus Spirillum. 



