80 MANUAL OF HUMAN PROTOZOA 



paratively large rounded body, stained red with Giemsa's 

 stain. But in ordinary films, the body may be flattened to 

 various extents, and the nucleus also becomes flattened and 

 is of various shapes. The blepharoplast is stained more 

 deeply, and is an oval or elongate body, much smaller 





\ 



Fig. 20, Leishmania donovani. x 1150. (original) 

 1-3. Forms found in a stained spleen puncture film. 



1. An infected polymorphonuclear leucocyte. 



2. Leishmania bodies scattered in the blood plasma. 



3. A large endothelial cell heavily infected by the organism. 

 4-6. Flagellate forms which developed in the first five days 



of cultivation in blood-agar medium. 



than the nucleus. When the smear is deeply stained, there 

 may be seen a short thread extending between the blepha- 

 roplast and the periphery. This is the rhizoplast. The num- 

 ber of parasites in a host cell varies greatly. A newly 

 invaded host cell may show a single parasite, while host 

 cells with 200 or more parasites are sometimes seen, this 

 being a result of repeated binary fission. No flagellate forms 

 occur in the human host. Dogs suffer also infection by 

 this organism. 



