88 MANUAL OF HUMAN PROTOZOA 



forms (Fig. 22, 2). In life, amoeboid movement is clearly 

 visible. The infected cells may be a bit larger, stain slightly 

 paler, and begin to show fine red dots known as Schliffner's 

 dots (Fig. 22, 3, 4). 



3. Grown schizonts. In about another 20 hours, the 

 schizonts (Fig. 22, 5, 6) are considerably larger and occupy 

 about two-thirds of the erythrocytes which may now be 

 as much as 12n in diameter and distinctly paler than un- 

 infected erythrocytes. Schliffner's dots appear to increase. 

 The schizonts are of irregular outline, enclosing one or 

 more large vacuoles and in addition pigment (haemozoin) 

 granules appear in the cvtoplasm. The haemozoin granules 

 are brownish in color in this species. A compact large 

 nucleus is easilv recognized, often located at a point in 

 periphery. 



4. Schizogonic or segmentation stage. The schizont nu- 

 cleus soon afterwards begins to divide (Fig. 22, 7) and the 

 division is repeated. Often some 16 daughter nuclei are 

 produced. The division of some of the nuclei are somewhat 

 irregular (Fig. 22, 8) so that there may be 12-24 nuclei in a 

 schizont (Fig. 22, 9). These multinucleate schizonts meas- 

 ure about 8-9|j in diameter. Presentlv around each of the 

 nuclei becomes difl^^erentiated a small cytoplasmic mass, 

 and thus many young schizonts or merozoites are produced 

 (Fig. 22, 10). The haemozoin granules present in the schiz- 

 ont assume a loose mass and are completely excluded 

 from the merozoites. The merozoites are somewhat irregu- 

 larly rounded and measure about 1.5|j in diameter. These 

 changes take place between 40 to 48 hours after the ma- 

 larial attack. The infected erythrocytes now rupture and 

 the merozoites are set free in the blood plasma. 



