DETECTION AND IDENTIFICATION 101 



that first advocated by Novy, MacNeal and Nicolle (or 

 N N N). This is prepared as follows: Dissolve 7 grams of 

 agar-agar and 3 grams of NaCl in 450 c.c. of distilled water 

 b\ heating. Place 5 c.c. of the mixture while still hot into 

 each test tube and apply a cotton plug. Autoclave the 

 tubes for 30 minutes under 15 lbs. pressure. Let the tubes 

 cool down to about 50° C, and add to each tube 15-20 

 drops of rabbit's blood with care to prevent bacterial con- 

 tamination. The tubes are rapidly evolved to bring about 

 mixture without forming bubbles, and sloped. When the 

 medium is solid, place rubber caps over the cotton plugs 

 and incubate the tubes at 37 °C. for 24 hours. The testing 

 blood is inoculated into the condensation water. 



