METABOLIC PATHWAYS IN MICROORGANISMS 



CARBOHYDRATE AND POLYOL OXIDATIONS 



Testing of various carbon compounds soon revealed that 

 several were inert, whereas others were susceptible to at- 

 tack to only a limited degree. Table 1.1 indicates the 

 extent of such oxidations. Where extensive oxidation oc- 



TABLE 1.1 

 Oxidation of Various Substrates by A. suboxydans 



O2 Consumption: ^tatoms/)umole 



Substrate 



Glycerol 



Dihydroxyacetone 



Ethanol 



Acetaldehyde 



Sorbitol 



Erythritol 



Pyruvate 



Lactate 



Acetate 



Ketoglutarate 



Malate 



Succinate 



Fumarate 



Citrate 



The systems contained 0.05M phosphate, 0.01 A/ MgCl2, 10-^A/ 

 DPN, and 10 mg. dry weight of washed cells. Volume = 2.8 ml., 

 pH = 6.0, temperature = 29° C. The substrate was tipped into the 

 main compartment containing dinitrophenol (lO^^Af) after 5 minutes' 

 preincubation. All values corrected for endogenous blanks, which 

 were about 0.1 fiatom oxygen//xmole substrate. 



