THE ACETIC ACID BACTERIA 



17 



TABLE 1.6 



Identification of Products of Sorbitol Oxidation 

 by A. suboxydans 



Pyridine Nucleotide 

 Added 



D-Fruc- 



tose L-Sorbose 

 Standard Standard 



0.93 

 -102 



0.69 

 -48 



1.00 

 -92 



0.73 



-42 



DPN TPN 



Position constant in 



phenol-HsO (4:1) * 

 la]'S (28), degrees 

 Crystal form of 



osazone (29) Rosettes Amorphous Rosettes Amorphous 



Mehing point of 



osazone (28), °C. 206 161.5-163 206 163 



* The position constant is the distance traveled by the compound 

 divided by the distance traveled by fructose. 



The reaction mixtures contained the following: 180 /umoles of 

 sorbitol, 10 jumolesof TPN or DPN, 3 ml. of CFE, 100 )umoles of TTZ, 

 500 )umoles of MgCl2, 1 mmole of tris buffer, pH 8.5. The total volume 

 was 10 ml.; temperature, 30°; time, 4 hours. 1 ml. of 50% TCA 

 was added, and the mixture v/as centrifuged and extracted with ether. 

 The [ajr? was calculated on the basis of sorbose and fructose deter- 

 mined both by the cysteine-H2S04 and resorcinol methods. [After 

 Cummins et al. (26).] 



ribitol, dulcitol, perseitol, glycerol, ethanol, acetaldehyde, 

 or 2-butene-l, 4-dioL Mannitol was oxidized as extensively 

 as fructose by whole cells (8.7 atoms of oxygen per molecule 

 substrate) but this may reflect only the easy conversion of 

 mannitol to fructose by this organism. Mannitol oxidation 

 has been found in our laboratory to be completely TPN- 

 specific and completely separable from the TPN-sorbitol 

 enzyme. 



The influence of pyridine nucleotide in guiding the oxi- 

 dation toward one end or the other of the sorbitol molecule 



