56 



METABOLIC PATHWAYS IN MICROORGANISMS 



TABLE 2.2 



Yields of Individual Atoms from Glucose and Gluconate: 

 B. subtilis Oxidations 



(Synthetic Medium) 



C^^02 Recovery 



If we now assume that re-formed fructose behaves like 

 administered glucose, Table 2.3 tells us that carbons A and 

 C of glucose will be recovered in COo to the extent of 60 

 and 67%, respectively. Multiplying 



88% X 67% X 60% = 35% 

 and multiplying 

 88% X 33% X 67% = 20% 



55% 



calculated recovery of C^'^O^ 

 from C-2 of gluconate 



This agrees well with the observed recovery of 54% from 

 C-2 of gluconate (Table 2.3). 



Formation of C^^Oo from 



