CARBOHYDRATE METABOLIC PATHWAYS 



69 



advances in refrigerated centrifugation, it was discovered 

 that the cell fractions discussed could be separated readily: 

 the nuclei and cell debris at centrifugal speeds of lOOOg 

 or less; mitochondria at 5000-20,000g; and microsomes at 

 25,000-50,000g-. When this was done, Lehninger found 

 (Table 3.1) (6) that whereas the nuclei, microsomes, and 



TABLE 3.2 

 Complete Oxidation of Members of the Citric Acid Cycle 



Oxygen Consumption 



Each flask contained 1.5 ml. of kidney mitochondria, 3 yumoles 

 ATP, 4 yumoles MgS04, 50 /xmoles phosphate buff'er, pH 7.2, and 

 substrate as indicated. 



After Green et al. (7). 



