78 



METABOLIC PATHWAYS IN MICROORGANISMS 



phofructokinase, all shared by either the pentose cycle 

 or the Entner-Doudoroff pathway. 



A second question to which we have sought an answer 

 is that of intracellular location of the respective enzymes. 

 Whereas we have seen how the Krebs cycle complex is 

 located in the mitochondria, Fig. 3.8 shows that this is 

 obviously not true of the enzymes dissimilating G-6-P or 

 R-5-P. Citrate added to this preparation is oxidized over- 

 70% of theory, thus assuring the integrity of the prepara- 

 tion. In Fig. 3.9, the disappearance of pentose and accumu- 



10 20 



30 40 50 



Time (minutes) 



60 



Fig. 3.9. Pentose cycle reactions in rabbit kidney tractions (non- 

 oxidative). 



M = mitochondria (residue, washed three times ^vith isotonic KCl) 

 S — kidnev fraction, sohible at 105,000fi^ 



