Contents 



CHAP. 



I. Introduction 



III. 



Technique 



(a) The Staitiitig of Bacteria 



(b) Hydrolysis Techniques 



PACE 



I 



(c) Differentiation Techniques ......... 8 



(d) The Romanou'shy Stains ......... 8 



(e) Simple Dyes ........... 9 



(f) Use of Enzymes, etc 9 



(g) Classical Cytoloj^ical Procedures . . . . . . . . lO 



(h) Cell Wall Stains I2 



(i) The Mounting of Material ......... 14 



(j) The Stainin(^ of Fla^ella ......... 16 



(k) Electron Microscopy . . . . . . . . . .16 



(1) Phase-contrast Microscopy ........ 



(m) Colony Preparations ......... 



(n) Summary .......... 



Surface Structures 



(a) The Cell Wall . 



(b) The Cell Membrane . 



(c) Development of Envelopes . 



(d) The Cell Wall of Myxohacteria 



(e) The Spore Coat 



(f ) The Nuclear Membrane 



(g) Slime Layers and Capsules . 

 (h) Flagella .... 

 (i) Summary 



17 

 21 

 21 



25 

 33 

 36 

 40 

 41 

 41 

 41 

 44 

 47 



IV. The Bacterial Nucleus 



(a) Historical 51 



(b) The Restin^T Nucleus 53 



(c) The Primary Vegetative Nucletis 62 



(d) Germination of the Resting Stage 64 



(e) The Spherical Vegetative Nucleus 65 



(f ) The Nucleus in Complex Vegetative Reproduction 67 



(g) The Nature of the Chromosome 72 



vii 



- '^5213 



