TECHNIQUE 



II 



(Reproduced from Experimental Cell Research) 



Fig. 



A GROUP OF BACILLUS FIXED AND STAINED BY VARIOUS METHODS 



(1) Diagram of the cytological structure of a group of bacilli at various stages of cell 

 division. The top-left bacillus is divided into four cells by complete cross-walls ; fission is 

 commencing. The top-right bacillus is divided by a complete cross-wall and subdivided by 

 cvtoplasmic septa alone. The lower bacillus has recently divided and has only two cells. 

 f.ZiV., cell wall ; ^.Z?., growing point ; 5., cytoplasmic septum preceding cross-wall ; s.e., septum 

 in earlv stage ; the darkly-stained junctions of the septa are growing points ; n, nucleus. 



(2) The same group demonstrated by a simple, basic dye. 



(3) Stained by Gram and over decolorised. 



(4, 5) Artefacts caused by unsuitable fixation. 

 (6) Sporulation appearances. 



