40 PROTOZOAN PARASITISM 



of illustrations of this body should lead immediately 

 to its recognition. 



For certainty in eliminating the possibility of End- 

 amoeha in the stool, more than these fresh immedi- 

 ately examined preparations is necessary. Perma- 

 nent stained specimens, by the haematoxylon 

 methods previously described, are recommended by 

 James (1927) and other experienced workers, on the 

 contention that amoebae may frequently be found 

 in these preparations when not in others and that 

 they may be more accurately identified. 



Consequently, the writer desires to recommend 

 such a measure when Endamoeba histolytica has not 

 been found in a suspicious case or when it has been 

 identified by any measure except actual counting 

 of characteristic nuclei in the cysts. It is freely 

 confessed, however, that his ideas do not follow this 

 far, in consideration of what is simple and therefore 

 practical and likely to be done in medical practice 

 outside of centers where the ultimate may be con- 

 sistently followed. 



It is emphasized that only a few are considered 

 competent to identify anything but the cyst, and 

 that familiarity with this stage should be stimulated 

 with medical laboratorians. 



Neither are cultures to be relied upon in any but 

 expert hands, although some have recommended 

 them for diagnosis in amoebic and other protozoon 

 intestinal infections. With the experienced and ex- 

 pert cultures may pick up an infection otherwise 



