52 PROTOZOAN PARASITISM 



for fertilizing garden stuff, fresh uncooked vegetables, 

 especially those kept moist, should not be eaten. 

 Possibly refrigeration of such articles for a period of 

 time, yet to be positively determined, sufficient for 

 the cysts to die, may render them safe for consump- 

 tion. 



EXPERIMENTAL INFORMATION 



Experimental infection of other animals with End- 

 amoeha histolytica from man has been done on nu- 

 merous occasions and has proven of great value in 

 determining the manner of occurrence of the infec- 

 tion and the nature of the disease produced by it. 



The most commonly used animal is the cat, kittens 

 being susceptible to the infection on injection of 

 material containing the amoeba by rectum, less so on 

 feeding the cysts. The lesions produced in such 

 a manner are similar to those of amoebic dysentery 

 in man, including the occasional occurrence of am- 

 oebic abscess of the liver. Since Hlava (1887) first 

 produced this experimental infection it has been 

 frequently done, the early experimenters being Kar- 

 tulis (1891), Kovacs (1892), Quincke and Roos 

 (1893), and Kruse and Pasquale (1894). 



Several workers, including Losch (1875), Hlava 

 (1887), Kruse and Pasquale (1894), and others, have 

 infected dogs. 



Guinea pigs have been experimentally infected by 

 Baetjer and Sellards (1914) and Chatton (1917). 



