104 PROTOZOAN PARASITISM 



istics of Councihnania, except that no budding was 

 seen, nor were chromosomes counted, which ingested 

 red blood corpuscles in culture tube. 



Artifacts of the cysts of amoebae are certainly very 

 common, although nothing resembling budding of 

 amoebulae is often seen. The general characteristics 

 of the species are common in amoebae identified as 

 Endamoeha coll at the present. As far as the num- 

 ber of chromosomes is concerned, Wenyon's difficulty 

 is no exception. The burden of proof still rests with 

 Kofoid and his adherents but if the budding process 

 was correctly observed it will undoubtedly be only 

 a matter of time until the controversy is cleared. 

 Further, if the number of chromosomes in the divid- 

 ing nucleus may be accurately counted, and, of course, 

 they will be, this will be a point of mathematical 

 calculation not subject to personal equation. That 

 time is not yet, however, certainly not for the average 

 experienced observer. The number of chromosomes 

 in the nucleus of amoebae, identified as such by Kof- 

 oid, in the lymph nodes of Hodgkin's disease and in 

 the bones of arthritis deformans, has been used as 

 the main point of identification of Endamoeha histo- 

 lytica in these instances. It is a very important 

 point of cytological distinction and further work will 

 be of great interest. 



