142 PROTOZOAN PARASITISM 



gation and experimentation of recent years. The 

 author (Lynch, 1915A and 1915B), Ohira and Nogu- 

 chi (1917), Boyd (1918), Pringault (1921), Hogue 

 (1921) and, more recently, numerous others have cul- 

 tivated it from various sources. Excellent results 

 have been obtained with the organism from the 

 mouth, vagina, intestine, and from urine (Trichom- 

 onas vaginalis), in liquid human blood serum diluted 

 with from four to ten parts of 0.5% to 0.9% sodium 

 chloride solution, Ringer's solution, and Locke's so- 

 lution, incubated at 37.5° C. The higher dilution 

 has been attended with more success in the case of 

 the intestinal trichomonad, the stronger solution 

 with that from the mouth. Those who are interested 

 in research in culture methods should consult the 

 original articles of those who have reported upon 

 it. 



AbiUty to produce prolifically varies in differ- 

 ent cases, and probably the associated bacterial 

 growth has much to do with this. All of these media 

 are slightly alkaline and usually become more so 

 with the age of the culture. The column of medium 

 should be rather deep, and the organisms may be 

 taken from the bottom of the tube with a capillary 

 pipette. The numbers are usually greatest at about 

 the third or fourth day but some may live for twelve 

 days or longer. Subculture should be planted on the 

 second or third day. 



Hinshaw (1927) gives the simplest medium he has 

 used as composed of 100 cc. of Locke's solution with 



