OF THE ALIMENTARY TRACT 161 



fully developed technique, showing the several struc- 

 tures but no particular nuclear detail. The detail 

 of structure may be found recorded by Hinshaw 

 (1926). 



As with other trichomonads of man, no cyst is 

 known. 



To find the organism it is necessary to take mate- 

 rial from beneath the gums or between the teeth. 

 This may be mixed on a microscopic slide with 0.9% 

 sodium chloride solution to a dilution suitable for 

 good vision when examined under a cover glass. The 

 low dry microscopic lens reveals its presence and it 

 may be studied under higher powers. 



Dark field examination makes a conspicuous ob- 

 ject of the organism, its flagella, undulating mem- 

 brane, blepharoplast, and food bodies standing out 

 as brilliant objects. When it slow^s dow^n the fla- 

 gella may be counted by this means, or by ordinary 

 high power examination when they have become very 

 slow or have ceased motion. 



For the best stained specimens thin smears of the 

 exudate of its habitat are made and stained as de- 

 scribed under examination for amoebae. 



Hinshaw (1928) produced experimental infection 

 of the mouths of dogs with pyorrhoea. 



Hogue (1926) found the thermal death point to be 

 45°C. 



Hinshaw (1927) found it survived suspended in 

 distilled water for 10 to 12 hours. 



