OF THE ALIMENTARY TRACT 173 



Here the nucleus, the marginal fiber and flagellum of 

 the cytostome, and the space between the anterior 

 pole and the uplifted end of the cyst shell may be 

 seen. 



Chilomastix mesnili was first cultivated by Boeck 

 (1921) in a medium composed of human blood serum 

 one part, Locke's solution four parts, with 0.25 gram 

 of dextrose to each 100 cc, incubated at 37 degrees 

 C. in 5 cc. amounts of the medium in small test tubes. 

 Boeck and Drbohlav (1925) and Thomson and Rob- 

 ertson (1925) report its cultivation on Boeck's L. E. 

 S. medium, as described under Eiidamoehea histo- 

 lytica. The writer has grow^n it in Hogue's ovo- 

 mucoid medium, Boeck's L. E. S., and Tanabe's 

 (1925) medium for trichomonads, composed of NaCl 

 0.7 gram, sodium citrate 1.09 grams, LoefHer's blood 

 serum, dehydrated, 0.5 gram, white of egg 2 cc, dis- 

 tilled water 100 cc. In making this medium the 

 salts are first dissolved, then the egg is added and 

 shaken vigorously, finally the dehydrated blood serum 

 is shaken wdth it. 



Success with these and other mediums is not al- 

 ways the same. As with trichomonads, bacteria are 

 uncontrollable and are unquestionably influential in 

 the success or failure in carrying on cultures. 



Division of the organism is by longitudinal bi- 

 nary fission, such as occurs in the case of the trichom- 

 onads. According to Kofoid and Swezy (1920) the 

 nucleus divides by mitosis. Following nuclear divi- 

 sion occurs the splitting of the cytoplasm, the 



