192 PROTOZOAN PARASITISM 



Wenyon and O'Connor has been confirmed by the 

 writer (1922). It has also been observed in this 

 country by Boeck (1924). 



The organism is from 4 to 7 or 8 microns in long- 

 est diameter, it being almost rounded or slightly 

 ovoid. It is very active in fresh preparation, swim- 

 ming jerkily and rotating at the same time. The 

 cytoplasm is alveolated and contains bacteria. It 

 anchors itself by a caudal process, as is the habit of 

 flagellates when feeding. 



The anteriorly directed fiagella move rapidly and 

 are not to be counted with certainty in an active 

 stage. At times they can be seen to come from a 

 minute knob, the blepharoplast. There is a re- 

 current flagellum which arises at the same place 

 passing posteriorly over the surface of the body, to 

 which it is adherent for about three fourths of the 

 body length, terminating in a free extremity from 

 the posterior quarter. In motion there is a slight 

 protoplasmic projection where this flagellum leaves 

 the body. The nucleus is not visible in the unstained. 

 The cysts are minute bodies, slightly smaller than 

 the trophozoite, and possess a nucleus in each end, 

 as seen in stained specimens. They are ovoid in 

 shape and have a definite cyst wall. 



In preparations stained by Wright's blood film 

 stain the fiagella could be observed and counted. In 

 iron-haematoxylon preparations the nucleus was 

 seen at the base of the fiagella. It was ovoid and 

 had a central karyosome and definite rim. 



