80 



Perspectives in Microbiology 



A noteworthy feature of this mechanism is that most 

 of the reactions occur only while the carboxyl groups of 

 the acids are in combination with the coenzyme. The rea- 



>CH3COSCoA + CH3CH2CH2COOH 



:2rCH3CH2CH2C05CoA + CH3COOH 



I 1 2H 



CH3COSC0A -jjj X 



+ l<- >CH2= CHCK2CC;CoA:;i=±CH3CH = CH COS Co A 

 CH,= CHCH,cJ ^ 1 , ■ 



CH,COOH 



IK 



+ H2O 



CH3CHOHCH2COSC0A 



IT 



± 2 H 



>2CH3COSCoA ,^ 



CH3COCH2COSC0A 



+ Co ASH 



H3PO4 



Co ASH 



/ 



CH,COOPOiH 



CH3CH2OH <.n3cuuru3n2 



FIGURE 7. Scheme of Fatty Acid Oxidation by Clostridium kluyveri 



son for this is not known, although it is probable that the 

 large coenzyme molecule serves as a tool for attaching the 

 substrate to the right part of the enzyme surface. Similar 



