THE CHOLERA VIBRIO 119 



practically spherical. It will be noted also from this figure that 

 the asymmetrical cells are not classified; that is, cells with terminal 

 bulgings, for instance, may be found at almost any position in the 

 column, their position being determined by their relative plumpness 

 or thinness, rather than by their asymmetry. 



It will be noted that during the lag phase practically no varia- 

 tion in the cells occurred. The cells used for inoculation consisted 

 in part of typical vibrio forms, and in part of small oval and coccoid 

 cells with some irregular budding and branching cells. With the 



L2 15 25 36 hS 72 qS 120 iGz 2Uc 



Hours. 



Fig. 33. Representative Cells from a Culture of the Cholera Vibrio at 

 Different Stages of Growth. 



initiation of growth at six hours a definite alteration in form occurred, 

 the majority of the cells being larger and relatively straight. These 

 forms persisted during the period of active growth, but with the 

 cessation of growth at twelve hours the cells became more slender 

 and more curved, and during the resting period these typical vibrio 

 forms were predominant. Beginning at thirty-six hours there ap- 

 peared more and more irregular cell forms of the type which I have 

 designated asymmetrical. Without any measurements, then, one can 

 clearly distinguish in this graph the occurrence of three distinct types 



