49. Bacillus 



Thirty-eight cultures, representing twenty-one species, of the 

 genus Bacillus were studied morphologically. The identity of the 

 cultures was accepted as labeled by the donors. The majority of 

 the cultures were from the R. N. Smith Collection and were re- 

 ceived from William B. Haynes of the Northern Regional Research 

 Laboratory, Peoria, Illinois. Several cultures were from Kenneth 

 Burdon, University of Texas. With the exception of Bacillus 

 pasteurii, which required addition of 1% urea to the medium, all 

 cultures grew readily in simple peptone broth. Only one thermo- 

 phile. Bacillus stearothermophilus, was studied and this was in- 

 cubated at 55° C. 



Fig. 52. a. Bacillus subtilis, NRRL B-642. Typical normal and coiled 

 flagella. 



b. B. subtilis, NRRL B-642. This is a somewhat unusual organism for this 

 species, showing two normal and two curly flagella. 



c. B. pumilus, Burdon Ba 7(5). Normal and coiled flagella. 



d. B. megaterium, NRRL B-349. Normal flagella. This strain also showed 

 some coiled flagella. 



e. B.cereus, Burden Ba 2(7). Normal flagella. Flagella of two distinctly 

 different wavelengths were observed in this culture. The shorter wavelength 

 flagella had a wavelength about 1.6 microns which is greater than tvpical curly 

 flagella. 



f. g. B. macerans, NRS 1093. In f is shown the normal and in g the curly 

 variant of this strain. 



h. B. macerans, NRRL B-171. This strain had a unique flagellation. The 

 flagella are short, stiff^, and with a very short wavelength — compare with g. 



i. B. macerans, NRRL B-172. Still a difi^erent picture of B. macerans show- 

 ing lightly stained soma with normal flagella on one individual and curly 

 flagella on the other. The normal flagella have a shorter wa\elength than 

 those of strain 1093 shown in f. 



j. B. pohjmyxa, NRRL B-173. Normal flagella of somewhat irregular 

 curvature. 



124 



