butylicum group. Straight flagella were observed only in Clos- 

 tridium aerofoetidum, McClung 1148, which showed a few 

 individuals with straight flagella. Several flagellar variants were 

 observed in Clostridium tetanomorphum as illustrated (Figs. 54, 55, 

 and 56). 



Fig. 54. a. Clostridium difficile, McClung 871. The three strains studied 

 showed an identical picture. Note the exceptionally short wavelength, shorter 

 than that of curly flagella on most bacteria. 



b. C. tetani, McClung 148. Two strains studied showed practically an 

 identical picture. The slides showed only normal flagella with the shortest 

 normal wavelength of all Clostridia studied, excepting C. difficile which may 

 have been curly variants. The flagella of C. tetani appear to be very stiff 

 with relatively large amplitude. They are very characteristic and practically 

 diagnostic. 



c. C. capitovale, McClung 1237. The two strains stvidied were identical 

 and showed normal flagella only. The flagellar wavelength is relatively short 

 and amplitude relatively great. Flagella give impression of being rather 

 delicate. 



d. C. noviji, McClung 151. Normal peritrichous flagella of rather large 

 amplitude. The flagellation resembles that of C. tetani except for greater wave- 

 length. A few curly types seen. 



e. C. botulinum, McClung 662. Normal flagella. Two of the tliree strains 

 studied were atrichous. The strain pictured showed a few partly curly 

 flagella. 



f. C. septicum, McClung 1019. This is a medium length filament, many 

 were several times this length, and characteristic of C. septicum. The flagella 

 pictured are of normal type with rather short wavelength and small amplitude. 

 In organisms other than that pictured curly flagella were interspersed among 

 the normal. 



g. C.felsineum, McClung 638. The organism pictured is typical of strain 

 638, with normal flagella which stained readily. However, strain 639 showed 

 more a picture resembling C. acetobutylicum and the flagella were difficult to 

 stain. Further study is necessary to definitely elucidate the flagellar character- 

 istics of this species. 



h. C.chauvoei (feseri), McClung 1436. Normal flagella. No variants ob- 

 served. 



i. C. centrosporogenes, McClung 136. Normal flagella. With the exception 

 of a single curly flagellum all flagella seen in this culture were normal. 



j. C. hifennentans, McClung 435. Only normal flagella observed on both 

 strains studied. 



k. C. lentoputrescens, McClung 998. Nornial flagella. The one strain 

 studied showed excellent flagellation with all flagella of normal curvature. 



132 



