ENZYMIC DEGRADATION AND BIOSYNTHESIS 79 



TABLE 25 



Optical Configuration of Alanine Samples Obtained from a 

 Uridine Nucleotide and from the Cell Wall of Staphylococcus 



aureus 



Alanine Samples % L-alanine % D-alanine 



Reference 65. 



TABLE 26 



Requirements for the Enzymatic Synthesis of 

 UDP-GNAc-Lactyl-(L)Ala-(D)Glu-(L)Lys 



Experiment 1: Experiment 2: 



System Cpm Nucleotide Added Cpm 



Complete 2620 UDP-GNAc-lactyl-ala-glu 5080 



-UDP-GNAc-lactyl-ala-glu UDP-GNAc-lactyl-ala 



-ATP UDP-GNAc-lactyl-ala-glu-lys 6 



-Mg+ + UDP-GNAc-lactyl-ala-glu-lys 



ala-ala 



In Experiment 1 various components were omitted from the 

 complete incubation mixture, which contained buffer, MgClg, 

 Ci4-lysine, ATP, UDP-GNAc-lactyl-(L)ala-(D)glu, and purified en- 

 zyme. In Experiment 2 other uridine nucleotides were substi- 

 tuted for UDP-GNAc-lactyl-(L)ala-(D)glu. Data are expressed as 

 cpm of Ci4-lysine incorporated into nucleotide, measured as char- 

 coal-adsorbable radioactivity. 



Reference 76. 



