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DISCUSSION 

 M. V. Edds, Jr., H. A. Went, A. C. Giese 



Dr. Edds (Brown University): Would you care to speculate on the mech- 

 anism responsible for concentrating and ordering the precursor molecules in 

 the spindle? 



Dr. Went: There is evidence, as I have indicated, that the precursor 

 molecules are rather widely distributed throughout the cytoplasm of the un- 

 fertilized egg, but we know nothing regarding the forces that align the pre- 

 cursor molecules into an orderly array. It is fairly clear that the centriole is, in 

 some unknown manner, responsible for this orientation. Experiments with 

 model systems (Anderson, 1956) show that one can get precipitation of pro- 

 teins in solution radiating from a point which can be analogized to the cen- 

 triole. Aggregation and ahgnment of macromolecules in model systems have 

 been thought of in terms of surface charges and changes in their distribution 

 or strength, conferring preferential aggregation patterns on the macromole- 

 cules. However, any resemblance between precipitation patterns observed in 



