210 MACROMOLECULAR COMPLEXES 



differential centrifugation in media of differing density, preparations 

 of isolated cell walls were obtained by Falcone and Nickerson 

 ( 1956 ) that were entirely free of intact cells and other particulate 

 cellular matter. Cells from starch-free pound cakes of bakers' yeast 

 (Anheuser-Busch) or laboratory-grown C. albicans were suspended 

 in water or in a 5 per cent aqueous solution of dithioglycol ( 150 g 

 yeast: 50 ml solution) and were disrupted in the cold by agitation 

 in a Waring Blendor with glass beads, according to the method of 

 Lamanna and Mallette (1954). More than 90 per cent breakage of 

 cells was achieved after 90 minutes' agitation. 



n-BUTANOL-ACETIC ACID-WATER 

 (a) 



Fig. 2. Two-dimensional chromatograms of amino acids obtained on acid 

 hydrolysis of isolated, clean cell walls (a) of Candida albicans, normal strain 

 582, and (b) of C. albicans, selenite-resistant mutant strain RM806, grown on a 



