POLYSACCHARIDE-PROTEIN COMPLEXES 



217 



glucaii component of the wall; in the latter, the scar results from 

 the circular ordering of fibrillar components into a highly crystalline 

 pattern. The biochemical and mechanical sequences that operate 

 during the budding process, and which bring about the circular 

 orientation of fibrils in an otherwise highly disordeied fibrillar net- 

 work, have been discussed in detail elsewhere ( Nickerson and Fal- 

 cone, 1959; Falcone and Nickerson, 1959). 



Evidence for the existence of a crystalline ringlike region in the 

 living cell at the juncture of bud and mother cell can be seen ( Fig. 

 7) from the work of Currier (1957) with aniline blue fluorescence 



Fig. 8. Electron micrograph of isolated cell wall of bakers' yeast. Note 

 difFerence in appearance between surface of outer layer and granular inner 

 surface of wall. 



