220 



MACROMOLECULAR COMPLEXES 



whereas GMP-II is responsible for the major peak observed .in 

 earher sedimentation diagrams. On prolonged centrifugation, the 

 GVIP-II diagram reveals the presence of two similar components 

 (Fig. 12). 



Fig. 11. Sedimentation pattern of glucomannan-protein I (GMP-I) in analyti- 

 cal ultracentrifuge. Material is evidently of low molecular weight. 



Fig. 12. Sedimentation of GMP-II in ultracentrifuge. Note correspondence 

 to pattern of peak shown in Fig. 3. On prolonged centrifugation, evidence for 

 two components is seen. 



Analysis of Polysaccharide-Protein Complexes. Lipid-free wall 

 preparations, subjected to the fractionation procedure shown in Fig. 

 4, yielded recoveries of the individual complexes from the various 

 yeasts as listed in Table 1. The glucan-protein fraction may account 



TABLE 1 



Polysaccharide-Protein Complexes of Yeast Cell Walls 

 in Per Cent * 



Organism Glucan-Protein GMP-I GMP-II 



Bakers' yeast 41.6 13.6 34.7 



S. cerevisiae 18.29 28.3 55.8 11.9 



C. albicans 582 47.4 3.0 27.2 



C. albicans me 45.0 2.1 34.3 



" Data expressed as percentages of lipid-free wall recovered when fractionated 



according to scheme shown in Fig. 4; based on data of Kessler and Nickerson ( 1959). 



