Electromclric and Other Studies of Cytochrome c 381 



In the cytochrome c group, the hgands to the 5th and 6th co-ordinate 

 positions are attached to the apo-protein. It is conceivable that owing to 

 differences between the natural apo-protein structures or to modifications 

 which bring about perhaps some unfolding of these structures, there is 

 variation in the strain and in consequence of the stability of the ligand 

 bondings. In such cases the resultant alterations in values of Kq and Kj^ 

 would be reflected in the E'q values. 



A cknowledgements 



We wish to thank Dr J. O'Hagan for details of myoglobin separation and 

 Mr E. Matthaei for the photograph (Fig. 5). 



We are indebted to the Australian National Health and Medical Research 

 Council for a grant towards the cost of the investigation. 



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