Table 6. Metal content of purified nitrate reductase 



* Dialysis was against deionized water. 

 i Internal standard method with Pd and V. 

 X Visual method. 



Table 7. Content of flavin in nitrate reductase 



* The sample used was the same preparation as in Experiment 1 

 but with preliminary digestion by crystalline chymotrypsin (8% of 

 weight of NaR) for 3 hr at pH 8-15, 37°C. 



t This value was obtained by D-amino acid oxidase assay. 



Table 8. Comparison of electron donors for purified nitrate 

 reductase activity 



* For these one-electron donors, the figures should be halved for the 

 cpmparison on the basis of nitrite-producing rate. 



t These true figures are known to be much less than that for reducad 

 phenosafranine. 



