Aiitoxidutiun of Flavocytoclnonie b^ 



527 



Effect of the Oxygen Pressure 



Figure 3 shows the effect of the oxygen pressure on the reaction rate. It 

 appears that the reaction rate is larger when the oxygen pressure is increased. 



Asymptote 



Fig, 



2 4 6 8 10 



l/pxIQ-" 

 4. Lineweaver and Burk plot of the ex- 



FiG. 3. The effect of the oxygen pressure 



on the rate of the reaction. Abscissa: perimental data of Fig. 3. Abscissa: the re- 

 time in min. Ordinate: oxygen con- ciprocal of the oxygen pressure (in mm Hg). 

 sumption. The number near each tracing Ordinate: the reciprocal of the oxygen con- 

 shows the oxygen pressure in mm Hg. sumption (//I. in 15 min). 

 Enzyme added: 17m/<moles. Other con- 

 ditions as in Fig. 1. The line marked 

 'asymptote' corresponds to the theoretical 

 consumption for the oxidation of lactate 

 present. 



Figure 4 is a plot according to Lineweaver and Burk. The oxygen pressure at 

 wliich, under the conditions of the experiment, the rate is half-maximal, is, 

 according to this figure, 570 mm Hg. 



Effect ofpH 



Figure 5 shows the effect of pH on the oxidation rate. The rate is maximal 

 around neutrality. It decreases clearly when the solution becomes more acid. 



The Effect of Ionic Strength 



Figures 6 and 7 show the effect of the ionic strength. The ionic strength was 

 calculated according to the equation 



CO = I 2///jZ^ 



