Action of Yeast Lactate Dehydrogenase 



539 



Enzyme concentration x 10^ M 



Fig. 4. Relationship between the rate and the concentration of enzyme. 

 Curve 1, ferricyanide; curve 2, methylene blue. Experiments were carried 

 out at 25°C and pH 7-2. 



0-5 



1-0 



20 



Concentration -of dye x lO^M 



Fig. 5. Relationships between the rates and the concentration of dyes. 

 Curve 1, 2:6-dichlorophenolindophenol; curve 2, thionine; curve 3, methylene 

 blue; curve 4, toluylene blue. Substrate concentration, 10 mM. Experiments 

 were carried out at 25°C and pH 7-2. 



