Various Forms of Yeast Lactate Dehydrogenase 551 



yeast lactate dehydrogenase was a precursor of the aerobic L-lactate-cyto- 

 chrome c reductase. The presence of D-lactate cytochrome c reductase in 

 aerobic yeast (Nygaard, 1960) lends support to this hypothesis and suggests 

 that D-lactate cytochrome c reductase is an intermediate in the transfor- 

 mation. The extreme salt-sensitivity of D-lactate cytochrome c reductase 

 recalls the extreme salt-sensitivity of L-lactate cytochrome c reductase as 

 reported by Boeri, Cutolo, Luzzati and Tosi (1955) and by Boeri and Tosi 

 (1956). The fact that L-lactate cytochrome c reductase isolated from our 

 yeast is insensitive to salt suggests that more than one form of this enzyme 

 can be isolated from aerobic yeast. One of the forms could be an inter- 

 mediate in the development of the other. 



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