82 DIFGOMANUAL 



To rehydrate the medium dissolve 22 grams of Bacto-Veal Infusion Broth in 

 1000 ml. distilled water. Distribute in tubes and sterilize in the autoclave for 

 15 minutes at 15 pounds pressure (121°C.). The final reaction of the medium 

 will be pH 7.3. 



One pound of Bacto-Veal Infusion Broth will make 20.6 liters of medium. 

 1 Standard Methods for the Examination of Dairy Products, gth Edition: 141: 1948. 



BACTO 



PPLO ENRICHMENT BROTH (B410) 



DEHYDRATED 



Beef Heart, Infusion from 50 g. 



Bacto-Peptone 10 g. 



Sodium Chloride 5 g. 



Bacto-Crystal Violet 0.01 g. 



Bacto-PPLO Enrichment Broth is prepared according to the formula sug- 

 gested by Morton, Smith, Williams, and Eickenberg,^ for the selective enrich- 

 ment of pleuropneumonia-like organisms (PPLO). This medium is designed to 

 facilitate the isolation of PPLO from clinical specimens. It is also useful as an 

 aid in the purification of PPLO colonies contaminated with other microorgan- 

 isms. The growth of both Gram-positive and Gram-negative organisms is sup- 

 pressed, while pleuropneumonia-like organisms develop. Following inoculation 

 with clinical specimens or with bacterial contaminated agar block colonies of 

 PPLO, incubate at 37°C. for 36-72 hours and then plate on PPLO Agar, con- 

 taining 1 per cent Bacto-PPLO Serum Fraction, or 25 per cent Bacto-Ascitic 

 Fluid. Inoculation of the plates is accomplished by transferring a drop of the 

 enriched culture to the plate and spreading it uniformly over the surface of the 

 medium. 



To rehydrate the medium, suspend 21 grams of Bacto-PPLO Enrichment 

 Broth in 1000 ml. of distilled water. Sterilize in the autoclave 15 minutes at 15 

 pounds pressure (121°C.). To each liter add 2.85 ml. of Bacto-Chapman Tellu- 

 rite Solution to give a concentration of 1-35,000 of potassium tellurite. Add 25 

 per cent Bacto-Ascitic Fluid and tube in sterile tubes under aseptic conditions. 

 The final reaction of the unenriched medium will be pH 7.8. 



One pound of Bacto-PPLO Enrichment Broth will make 21.6 liters of unen- 

 riched medium. 

 ij. Dental Researchj 30:415:1951. 



BACTO 



THIOL MEDIUM (B307) 



DEHYDRATED 



Proteose Peptone No. 3, Difco 10 g. 



Bacto- Yeast Extract 5 g. 



Bacto-Dextrose 1 g. 



Sodium Chloride 5 g. 



Thiol Complex 8 g. 



Bacto-Agar 1 g. 



/7-Aminobenzoic Acid 0.05 g. 



Bacto-Thiol Medium is recommended for culturing organisms from body 

 fluids and other materials containing penicillin, streptomycin and sulfa drugs. 



