DEHYDRATED CULTURE MEDIA 83 



This medium does not contain thermolabile factors and accordingly may be 

 sterilized in the autoclave. Bacto-Thiol Medium has the ability to inactivate 

 penicillin, streptomycin and sulfa drugs. Luxuriant growth of staphylococci and 

 other test organisms will be obtained from dilute inocula in 24 hours in 10 ml. 

 of Bacto-Thiol Medium to which has been added 100 to 1000 units of penicillin, 

 1000 to 10,000 units of streptomycin, or sulfonamides. Bacto-Thiol Medium con- 

 tains 0. 1 per cent Bacto-Agar to maintain an" OR potential conducive to growth 

 of the strictest anaerobes, microaerophiles, and aerobic microorganisms, without 

 special seal. 



The medium should be distributed in tubes or containers to give a depth 

 of at least 60 mm. for neutralization of penicillin. For the neutralization 

 of streptomycin, the medium may be employed in tubes or in shallow layers in 

 flasks. Neutralization of antibiotics and cultural response with Bacto-Thiol 

 Medium Is best when freshly prepared, or less than 4 days old. 



Bacto-Thiol Medium enriched with 1 per cent Bacto-Supplement B has proven 

 an especially valuable medium for blood culture. Broom^ reported excellent re- 

 sults with the enriched Thiol Medium in the isolation of Hemophilus influenzae 

 and meningococci. Huddleson- reported this medium satisfactory for the isola- 

 tion, cultivation and maintenance of stock cultures of Vibrio fetus. Cultures re- 

 mained viable in Thiol Medium for at least 150 days without transfer. 



To rehydrate the medium, suspend 30 grams of Bacto-Thiol Medium In 1000 

 ml. freshly distilled water. Heat to boiling to dissolve the medium completely. 

 Distribute in tubes or flasks as indicated above and sterilize In the autoclave for 

 15 minutes at 15 pounds pressure (121°C.). The final reaction of the medium 

 will be pH 7.2. 



*^ Personal Communication, 1948. ^ J. Bact., 56:508:1948. 



BACTO 



THIOL BROTH (B434) 



DEHYDRATED 



Bacto-Thiol Broth has the same composition as Bacto-Thiol Medium except 

 it contains no agar. It is prepared for laboratories requiring a medium having 

 the properties of Thiol Medium except without agar. In rehydrating Bacto- 

 Thiol Broth 29 grams are used per liter. 



The final reaction of the medium will be pH 7.2. 



BACTO 



KRAGKE BLOOD CULTURE MEDIUM (B41) 



DEHYDRATED 



Beef Heart 165 g, 



Beef Brain 55 g 



Proteose Peptone, Difco 10 g 



Bacto-Dextrose 10 g, 



Sodium Chloride 4 g 



Sodium Citrate 1 g 



Disodium Phosphate 2 g 



Bacto-Kracke Blood Culture Medium is recommended for the culturing of 

 organisms from the blood in bacteriemias. The medium may also be used for 

 maintaining cultures isolated from the blood and for carrying stock cultures. 



